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KMID : 0352519810180020397
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Korea Univercity Medical Journal
1981 Volume.18 No. 2 p.397 ~ p.404
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Attenuation of Japanese Encephalitis Virus in Tissue Culture Cells
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Abstract
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Japanese encephalitis (JE) is an arbor viral disease and it has been prevalent for last 50 years in the Far East. Very recently, there were several large epidemic of the encephalitis with 30% mortality both in the countries of South East and Mid-West Asia.
The formalin inactivated vaccine derived from mouse brain inoculated with JE virus has been used in human for prevention of the disease in Asia since 1940. However, the vaccinee under age of 12 in the endemic areas of the disease has to receive the booster dose of the vaccine every year for maintaining high level of neutralizing antibody against the virus. It is hoped to develope an attenuated strain of JE virus in tissue culture cells for preparation of live vaccine which could be used to produce a long lasting immunity against JE.
The author inoculated two different strains of JE virus into normal and malignant cell cultures to propagate serially. The cytopathic effect in cell cultures and the pathogenicity of the virus in albino mice were observed and the results were as follow.
1. The cytopathic effect of the virus to the Maben, clonal liver and conjunctiva cells
were not observed.
2. The pathogenicity of the virus which were passaged serially in clonal liver and
conjunctiva cell cultures against albino mice was decreased significantly.
3. However, the virus propagated serially in Maben cell culture did not loose the
pathogenicity against albino mice.
The above results indicate that attenuation of JE virus could be developed in tissue culture cells.
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KEYWORD
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